Although water hyacinth is an adventive aquatic plant that threatens the japanese ecosystem, its high growth rate may be a benefit in terms of its potential as a raw material for the production of bioethanol. Water hyacinth was pretreated with different concentration of sulfuric acid, detoxified with caoh and naoh and then fermented using zymomonas mobilis cp4 a. Such research includes methods and conditions of pretreatments. Separated hydrolysis and fermentation of water hyacinth. Three different kinds of pretreatment methods, including microbial pretreatment, microbial combined dilute acid pretreatment, and microbial combined dilute alkaline pretreatment, were investigated for water hyacinth degradation. Previous research on ethanol production from water hyacinth revealed that it can be successfully converted to ethanol without much difficulty. This lignocellulose can more easily be bioconverted by enzymatic means to fermentable sugar, thus resulting in an. Water hyacinth was pretreated with different concentration of sulfuric acid, detoxified with caoh and naoh and then fermented using zymomonas mobilis cp4 a recombinant strain. Production of bioethanol from eichhornia crassipes water. Ethanol is produced from water hyacinth, an aquatic weed plant, comprising of cellulose, hemicelluloses and lignin by a twostep method.
Pdf bioethanol production from water hyacinth hydrolysate by. Review on pretreatment methods and ethanol production from. Water hyacinth control and possible uses introduction water hyacinth eichhornia crassipes water hyacinth is an aquatic plant which can live and reproduce floating freely on the surface of fresh waters or can be anchored in mud. This is due to the decreasing amount of fossil fuels, alternative energy sources need to be. Much of the recent interest is in the use of ethanol as fuel. Production of ethanol from water hyacinth eichhornia crassipes by. Water hyacinth is low in lignin content with high amounts of cellulose and hemicellulose poddar et al. Water hyacinth is an aquatic weed listed as one of the most productive plants on earth. The present study was undertaken to screen the feasibility of using water hyacinths hemicelluloses as a substrate for alcohol production by microbial fermentation using mono and cocultures of trichoderma reesei and fusarium oxysporum with pichia. For the first time, il pretreatment with 1ethyl3methylimidazolium acetate emimac was used for wh, and the efficiency was compared with the. The economic feasibility of the valorization of water hyacinth for. Water hyacinth eichhornia crassipes martius is a monocotyledonous freshwater aquatic plant, belonging to the family pontederiaceae, related to the lily family. Water hyacinth eichhornia crassipes is a fast growing, perennial aquatic weed.
Water hyacinth is considered as one of most abundant lignocellulosic feedstock in thailand. Producing ethanol from water hyacinth through simultaneous. In the present study biofuel ethanol production was carried out from a potential substrate eichhornia crassipes water hyacinth by separate hydrolysis and fermentation. Sustainable biofuel production from water hyacinth. Water hyacinth has low lignin, which means the cellulose and hemicellulose are more easily converted to fermentable sugar thus resulting in enormous amount of utilizable biomass for the biofuel industry. Pdf ethanol production from cellulosic biomass could effectively increase using enhancers. Batch fermentation and simultaneous saccharification and fermentation ssf were. Further, water hyacinth grows at a very rapid pace and contains very high nitrogen content. One approach to effectively control the rapid expansion of water hyacinth is to use it as a feedstock in producing valuable goods.
Dec 15, 2008 one alternative is the production of methane gas by the fermentation of various types, of biomass. The utilization of water hyacinth as the feedstock for bioethanol production has a number of advantages. Cmc synthesis from water hyacinth cellulose using isopropyl alcohol, isobutyl alcohol, ethanol and water as reaction medium was observed 27. Fermentation and the preparation of ethanol production of. In favorable environmental conditions, an individual water hyacinth can produce 140 million daughter plants annually. One alternative is the production of methane gas by the fermentation of various types, of biomass. Ethanol production from the hydrolysate of water hyacinth pretreated with dilute acid by simultaneous saccharification and fermentation has. The efficiencies of ethanol production from the whb hydrolysate are very less and the maximal ethanol yield was 3. Optimization of xylose yield from water hyacinth for ethanol production using taguchi technique. This study investigated the possibility of enhancing bioethanol production by combined pretreatment methods for water hyacinth. Enhanced acid hydrolysis for bioethanol production from. Optimization of bioethanol production using whole plant of. Bioethanol is simply ethanol is a renewable energy source which is made by fermenting the sugar and starch components of plant. Efficient bioethanol production from water hyacinth.
Ethanol production from cellulosic biomass could effectively increase using enhancers. Two fungal strains aspergillus niger local isolate and aspergillus niger mtcc 2196 were used for hydrolysis of water hyacinth. Distillation removes the water content from fermented juices. For example, water hyacinth has been demonstrated to be useful to develop supercapacitors, for production of ethanol, and to improve the immune resistance of plants and animals. The high rate of propagation and easy availability of water hyacinth has made it a renewable carbon source for biofuel production. A regression model was built to optimize the fermentation factors according to response surface method in. Bioethanol fermentation from acidbasetreated water hyacinth. Bioethanol production from sugarcane bagasse using. Fermentation of sugar released by saccharification of lignocellulosic materials produce bioethanol which is a renewable energy source. The synthesis of cmc from water hyacinth cellulose by using various mixtures of. Water hyacinth was used as substrate for bioethanol production in the present study. Agro residues when used for ethanol production may address this problem to an extent, but the operation of largescale plants for cellulosic ethanol production still have several limitations, including high capital investment, technical knowledge, and the high transportation costs of feedstock. Pdf ethanol production from water hyacinth eichhornia.
Since the water content of fresh water hyacinth is 93. The present study, therefore, was carried out to screen the feasibility of using hexose and pentoseutilizing fungal strains trichoderma reesei, fusarium oxysporum,andpichia stipitis for the effective conversion of water hyacinth biomass into ethanol. Frontiers optimization of bioethanol production using whole. Feb 15, 2016 the utilization of water hyacinth as the feedstock for bioethanol production has a number of advantages. This study focuses on the bioethanol production from enzymetreated hydrolysate of acidbase pretreatment water hyacinth. A proposed alternative to miti gate the excessing water hyacinth problem is to use its biomass aiming the production of secondgeneration ethanol 2g, using. In addition to its contribution to energy diversification, the production of bioethanol using water hyacinth as a. Production of bioethanol from eichhornia crassipes water hyacinth. In dap, 1 g dried water hyacinth was mixed with 20 ml of 2% vv sulfuric acid, and autoclaved 121 c, 15 lb for 1 hour, followed by neutralization with concentrated naoh 510 m to ph of 45. The effect of isopropyl alcohol 2butanol mixed solvent on. Water hyacinth water hyacinth, eichhornia crassipes, is a floating plant an invasive nuisance planta, non grata in much of the world where it often jams rivers and lakes with tons of floating plant matter 4.
Production of ethanol from water hyacinth eichhornia. The plant would be built in the first year, the production would be started from the second year and the project time horizon is 16 years according to the lifespan of the major. Water hyacinth secondgeneration ethanol production taylor. Plant size ranges from a few inches to a metre in height. Combination of acid pretreatment and enzymatic hydrolysis was the most effective process for sugar production that resulted in the production of 402. Up to 96% ethanol can be produced in alcohol distillery. Water hyacinth eichhornia crassipes biomass as a biofuel. Enzymatic hydrolysis of water hyacinth biomass for the. Optimization of xylose yield from water hyacinth for ethanol.
Chemical pretreatment methods for the production of. Water hyacinth was pretreated with different concentration of sulf uric acid, detoxified with caoh and naoh and then fermented using zymomonas mobilis cp4 a recombinant strain. Research has been conducted for the cultivation of the fresh. The production yields of ethanol obtained from the acid hydrolysis of water hyacinth using different approaches has been studied. Ethanol has been part of alcoholic beverages for long time, but its application has expanded much beyond that during the 20th century. Some results revealed that using the sulfuric hydrolysis following by the bioconversion of c.
Sustaining ethanol production from lime pretreated water hyacinth. It has been selected as a substrate for producing ethanol at an economically feasible manner. Research open access sustaining ethanol production from. The optimal hyperthermal ht acid hydrolysis conditions were 8% wv slurry content, 200 mm h2so4, at 160. Water hyacinth is a promising plant for the production of bioethanol. Ssf of pretreated water hyacinth biomass whb with p.
Effect of various pretreatment methods on sugar and ethanol. The results show the potential of water hyacinth as feedstock for ethanol production however the high moisture content and other chemical analysis also favors the methane production. The mixture of cowdung and water hyacinth slurry has proven to produce more biogas than when used alone elshinnawi et al. Water hyacinth eichhornia crassipes and water lettuce pistia stratiotes l. The results show that in comparison with the active control approach of collection and land. Production of bioethanol as useful biofuel through the. Jun, 2012 although water hyacinth is an adventive aquatic plant that threatens the japanese ecosystem, its high growth rate may be a benefit in terms of its potential as a raw material for the production of bioethanol. Water hyacinth water hyacinth, eichhornia crassipes, is a floating plant an invasive nuisance planta, non grata in much of the world where it often jams rivers and lakes with tons of floating plant. Water hyacinth was pretreated with different concentration. Here, about 14 gl of bioethanol 185 mgg of dry material was produced by candida tropicalis y26 using production medium with water.
In simple sterilization, 18 g of water hyacinth 94. It is produced from the agricultural product such as corn, sugarcane, potatoes, rice, beetroot and recently using grapes, banana, dates and other wastes. Water hyacinth eichhornia crassipes was used as a feedstock for ethanol production. Eichhornia crassipes a potential substrate for biofuel. Enhancing bioethanol production from water hyacinth by new. Effects of acid, alkali, ionic liquid il, and microwavealkali pretreatments on cellulosic water hyacinth wh were investigated based on the total reducing sugars trs and ethanol production. Ethanol production, purification, and analysis techniques. The cost of raw material is a significant factor affecting the biomass to ethanol process and the availability of cheap sustainable biomass resources is a major issue world wide. Sustaining ethanol production from lime pretreated water. Ethanol production from the water hyacinth eichhornia crassipes by. Pdf ethanol production from candidate energy crops. Biogas production using water hyacinth eicchornia crassipes. Enzymatic hydrolysis of pretreated wheat straw produced reducing sugar yields mgg of sample of 423 and 487. Research open access sustaining ethanol production from lime.
These aquatic plants deplete dissolved oxygen in the. The present study was undertaken to screen the feasibility of using water hyacinth s hemicelluloses as a substrate for alcohol production by microbial fermentation using mono and cocultures of trichoderma reesei and fusarium oxysporum with pichia stipitis. Now it needs to find the technology to turn the result into a october 24, 2016 read full article. Ethanol fuel has a gasoline gallon equivalency gge value of 1.
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